Wide-field epifluorescence is the most straightforward method of fluorescence microscopy. Excitation light is directed through the objective lens to illuminate the sample and the same lens used to capture the emitted fluorescence. The emitted light can be looked at by eye or captured using a sensitive camera. Wide-field systems have the benefits of ease of use, speed and sensitivity, making them suitable for a broad range of different applications including live cell imaging, imaging tissue sections and imaging samples prepared in multi-well plates.
The Imaging Facility has several wide-field fluorescence microscopes, offering different capabilities and levels of automation.
4x, 10x, 20x, 40x, 60x, 100x objectives
Hamamatsu Flash 4.0 sCMOS camera
Nikon Ri2 colour camera
Full enclosure incubation (temperature and CO2)
Nikon JOBS (automated imaging and analysis)
10x, 20x, 40x, 63 objectives
Leica K8, Scientific CMOS camera
Leica LED8 illuminator
Full enclosure incubation (temperature, CO2, O2)
Hardware focus drift compensation
CoolLED pE-4000 illuminator
CoolLED pE-340fura illuminator
5x, 10x, 20x, 40x objectives
Fluorescence and colour brightfield imaging
Up to 7 fluorescence channels
Fully automated imaging
Capacity for up to 100 standard microscope slides
Integrated barcode reader